FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l06n01 ah3337a-042415.341000000090db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l06n01 ah3337a-042415.341000000090db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26460712
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG289861910.954425564965902TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3353200.044.559321
GCACACG3326700.044.30292511
TCGGAAG3369800.044.2290843
AGCACAC3343500.044.17542610
GAGCACA3346300.044.1747639
ACACGTC3337550.044.1551113
CACACGT3340250.044.14214712
AGAGCAC3352750.044.1045388
ATCGGAA3389200.044.0646132
CACGTCT3339000.044.0461814
ACGTCTG3341400.044.01252415
CGTCTGA3345100.043.9201316
CGGAAGA3400000.043.7875184
GTCTGAA3365150.043.6712317
GAACTCC3350250.043.64727421
TCGTATG3296700.043.6346645
TCCAGTC3332900.043.56575825
TCTGAAC3375750.043.4794618
ACTCCAG3350050.043.45943523
GAAGAGC3416350.043.4486586
Produced by FastQC (version 0.11.2)