FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l06n01 ah119k-042415.34100000009103.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l06n01 ah119k-042415.34100000009103.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11381144
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA549190.48254375834274654No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC447450.3931502843650867No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG284850.2502823969189741TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGGG52950.041.2902616
CCTCGGG53150.041.13488815
GGCGGTC65900.040.97324438
TCGGGGA68050.039.73046517
CCAGGCG67650.039.66478335
CAGGCGG68750.039.1373236
CCCAGGC68750.039.13255734
GTCTCCC68750.039.1119742
CTGACCC66900.038.78055240
CGTCCGC35000.038.6236933
CCTGACC67800.038.297339
AGGCGGT70550.038.26964637
GCGGTCT70700.038.12793439
ACGTCCG36700.037.4464832
TAAGCCG69650.037.29994616
CGGTCTC72550.037.12627840
GCACACG41150.036.951811
CCAGGGA70550.036.8270265
GGTCTCC73550.036.8052241
TCCTCGG60450.036.35312714
Produced by FastQC (version 0.11.2)