FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l05n01 ah7797i-042415.34100000009098.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l05n01 ah7797i-042415.34100000009098.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18351385
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG2201511.1996424248088087TruSeq Adapter, Index 16 (97% over 40bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA704130.38369311090143876No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC569750.3104670301451362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG259450.043.35470635
GTCCCGA256000.042.9294736
GCACACG276750.042.71215411
TCACCCG272350.042.28812830
ACACGTC279700.042.1732113
ACCCGTC271950.042.09988832
CCCGTCC270650.042.0458433
ACGTCTG281800.041.9078515
CACCCGT274900.041.75447531
CACGTCT282950.041.67278714
CACACGT283400.041.67022312
GATCGGA287200.041.624421
CCCGATC261250.041.6211138
CCGTCCC272950.041.397834
AGCACAC286000.041.36230510
CGTCTGA285750.041.30481716
TCCCGAT266300.041.15167637
CCGATCT266150.040.85483639
CGATCTC267100.040.81213840
GAGCACA291600.040.721259