FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l05n01 ah7797a-042415.3410000000907e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l05n01 ah7797a-042415.3410000000907e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25600015
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG416560.16271865465703828TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG69300.032.45787411
ACACGTC75750.029.7832213
ACGTCTG77250.029.20518915
CACACGT77750.028.9881612
CACGTCT81600.027.6203514
CGTCTGA84750.026.91255616
GATCGGA86600.026.3902591
AGCACAC88300.025.7285710
GAACTCC89550.025.4479221
GAGCACA93000.024.7422059
CAGTCAC89850.024.43847727
TCCAGTC94300.023.54794325
AGTCACA94000.023.14300728
CCAGTCA95800.023.0383326
CTCGTAT94600.022.94370344
AGAGCAC101500.022.6923128
CACATGT94950.022.67013731
TCACATG96450.022.36552230
ATCTCGT97650.022.32114242
GTCACAT97550.022.13711729
Produced by FastQC (version 0.11.2)