FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l05n01 ah7609a-042415.341000000090e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l05n01 ah7609a-042415.341000000090e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20323776
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC2063891.015505189586817TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG241200.041.99471311
GATCGGA250400.041.2999081
ACACGTC248950.040.5697313
ACGTCTG250300.040.4686415
CACACGT250800.040.35138712
CACGTCT251550.040.1056914
CGTCTGA252600.040.0288216
AGCACAC257050.039.46662510
GAGCACA259850.039.3250929
TATCTCG250400.039.1171839
CCGATGT251150.038.99627733
CAGTCAC257900.038.80227327
GTCACCG255300.038.7683429
GTATGCC256100.038.73361245
AGAGCAC264100.038.632548
TCGGAAG268250.038.5808533
TCCAGTC260650.038.54863425
AGTCACC259300.038.27853828
CCAGTCA261750.038.27489526
GAACTCC265500.038.0944521
Produced by FastQC (version 0.11.2)