FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l05n01 ah6408i-042415.34100000009061.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l05n01 ah6408i-042415.34100000009061.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15850426
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG2705381.706818479200496TruSeq Adapter, Index 14 (97% over 44bp)
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC165190.10421801912453331No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA161590.10194678679298588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG315950.043.1285711
GATCGGA326750.042.2883721
ACACGTC322100.042.2281513
CACACGT322600.042.22558612
ACGTCTG322250.042.1604315
CACGTCT322550.042.12042214
AGCACAC325800.041.91440610
GAGCACA327200.041.7472349
TCGGAAG332300.041.5083583
AGTTCCG316900.041.39867834
CGTCTGA328850.041.29375516
GAACTCC327100.041.28373321
CCGTATC317600.041.23625638
TATCTCG322150.040.97072241
CAGTCAC327000.040.94328727
GTTCCGT320150.040.92687235
TCCAGTC328150.040.86836225
CCAGTCA327800.040.836526
GTCACAG327700.040.70600529
AGAGCAC337100.040.581258
Produced by FastQC (version 0.11.2)