FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l05n01 ah6408a-042415.34100000009048.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l05n01 ah6408a-042415.34100000009048.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22894934
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG4952932.163330106127408TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG579600.043.139411
GATCGGA593400.042.956291
CACACGT588850.042.4579212
ACACGTC590200.042.38739413
ACGTCTG588750.042.38601315
CACGTCT590850.042.2150414
AGCACAC595900.042.0688510
GAGCACA600800.041.941799
CGTCTGA596550.041.8166316
AGAGCAC610350.041.3296748
GAACTCC597900.041.28260821
TCGGAAG616600.041.280173
TCCAGTC599550.040.8346925
CAGTCAC601900.040.80225827
CACAGTC595700.040.79395331
ATCGGAA626150.040.72962
CCAGTCA603200.040.5353826
GTCTGAA619050.040.46285217
AGTCACA607400.040.45316328
TCGTATG605050.040.36351445
Produced by FastQC (version 0.11.2)