FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l05n01 ah6309b-042415.3410000000902e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l05n01 ah6309b-042415.3410000000902e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18625790
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG342990.1841478938611463TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG58150.033.2663911
AGTCACG58750.031.55332428
GATCGGA62800.030.7256031
ACACGTC63200.030.46563113
CACACGT65550.029.40797212
ACGTCTG66000.029.2413115
GATCTCG62200.029.10699741
CGATCTC61200.029.03092440
CACGTCT67100.028.56050514
CTCGGGG17700.028.21195616
AGCACAC69550.027.8136710
CGTCTGA70300.027.48471816
GTCACGT67800.027.13904629
GAGCACA74400.026.3025999
CCTCGGG19100.026.2618315
CAGTCAC71900.025.97164727
GAACTCC73250.025.92083221
TCACGTG71300.025.86916730
CACGTGA71900.025.80874631
TCCAGTC74550.025.35036725
Produced by FastQC (version 0.11.2)