FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l05n01 ah6309a-042415.34100000008ff9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l05n01 ah6309a-042415.34100000008ff9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23886196
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG345400.1446023468952528TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG56200.032.81934732
CGTCCGC57050.031.93702733
GCACACG64800.030.72092611
AGTCACG66350.028.4818428
CACGTCC66350.028.06978631
GTCCGCA65400.027.89599634
CTCGGGG21850.027.79592116
GATCGGA73100.027.625571
ACACGTC72800.027.15944113
CCGCACA67700.026.97181936
CACACGT73650.026.876712
ACGTCTG74850.026.44603315
CACGTCT76250.025.95992314
TCACGTC72550.025.95148730
CGTCTGA76950.025.7827216
CATCTCG73200.025.65538641
TCCGCAC72800.025.07080735
CCTCGGG24300.024.90093615
GTCACGT76400.024.7328329
GGCGGTC29150.024.4871838
Produced by FastQC (version 0.11.2)