FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l05n01 ah119j-042415.341000000090ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l05n01 ah119j-042415.341000000090ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17929516
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC210030.11714203551283817TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG38000.029.065111
CTCGGGG16800.026.77954116
GGCGGTC21400.025.6724238
ACACGTC43400.025.65602713
CCTCGGG17450.025.65311215
GATCGGA43800.025.6019291
CACACGT44800.024.80406612
ACGTCTG45550.024.64305715
CCCAGGC23350.023.98958634
CACGTCT46900.023.83725714
CGTCTGA48050.023.40771516
CATCTCG44300.023.3800239
CCAGGCG24050.023.0207435
TCGGGGA24900.022.85515817
AGCACAC48900.022.81644210
CAGTCAC48450.022.4746727
GAGCACA52300.021.6762719
CAGGCGG26600.020.99099236
TCCAGTC53550.020.75464425
GTCTCCC27800.020.32849142
Produced by FastQC (version 0.11.2)