FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l05n01 ah119f-042415.34100000008fec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l05n01 ah119f-042415.34100000008fec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18190792
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC13834517.605226864228891TruSeq Adapter, Index 12 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA9192645.053457815360651No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC7572844.163007306113994No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC1643440.9034460951452802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1523500.044.52728311
GATCGGA1544400.044.471051
ACACGTC1526100.044.3644613
CACACGT1526950.044.3500812
AGCACAC1533100.044.27939210
CACGTCT1530250.044.2348414
GAGCACA1540650.044.2017069
ACGTCTG1533500.044.17274515
AGAGCAC1543350.044.1665278
TCGGAAG1555600.044.1369553
CGTCTGA1535300.044.08127616
ATCGGAA1562550.043.976632
CCAGGGA970050.043.9647185
TCCAGGG971050.043.9379734
CGGAAGA1564350.043.8441934
GTATGCC1518000.043.8140245
GAACTCC1539750.043.79836721
CTGACCC970100.043.7691640
TAAGCCG973400.043.75272416
CCTCGGG815750.043.73772415
Produced by FastQC (version 0.11.2)