FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l05n01 ah119d-042415.341000000090b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l05n01 ah119d-042415.341000000090b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20038670
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC13570226.772016306471437TruSeq Adapter, Index 5 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA3937711.9650555650649468No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC3332131.662849879757489No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC697500.3480769931337758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1508550.044.5354921
GCACACG1490950.044.43580611
ACACGTC1491700.044.34527613
CACACGT1494200.044.3135612
TCGGAAG1514500.044.258523
CACGTCT1493250.044.23737314
GAGCACA1504700.044.2201049
ACGTCTG1493900.044.21867415
ATCGGAA1521000.044.1521952
AGCACAC1502500.044.15110810
CGGAAGA1527550.043.82744
GTCTGAA1507000.043.8270117
GTATGCC1487200.043.69399645
TCCAGTC1492600.043.68718725
CCAGTCA1490450.043.67322526
GATCTCG1476700.043.6538839
TCTGAAC1510550.043.63466618
CAGTCAC1495550.043.62035427
CGTCTGA1513250.043.58454516
GTCACAC1496050.043.5445929
Produced by FastQC (version 0.11.2)