FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l05n01 ah119a-042415.34100000008fdf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l05n01 ah119a-042415.34100000008fdf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34354201
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC2046680.5957582887752214TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG256050.039.9787811
GATCGGA274250.038.0355221
CACACGT269350.037.9963512
ACACGTC271000.037.84800713
ACGTCTG270650.037.72313715
CACGTCT273700.037.3513214
CGTCTGA277100.036.9180716
ACTGACC270600.036.66462732
AGCACAC281300.036.5581810
GAGCACA285000.036.15369
GTATGCC278850.035.62883445
CAGTCAC282750.035.5831727
GAACTCC288400.035.35057421
TCCAGTC286600.035.2939825
CGTATGC284650.034.88355344
CCAGTCA290450.034.7796726
TCGGAAG302950.034.5103153
AGAGCAC299500.034.4858178
CTCGTAT292100.033.99005542
ATCTCGT293500.033.8753440
Produced by FastQC (version 0.11.2)