FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l04n01 foxflacz18hpf2.34100000008f8f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l04n01 foxflacz18hpf2.34100000008f8f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39001573
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT363750.016.3159941
TTTGTGT767200.015.91122
AACTTTG379650.015.6294282
CTTTGTG872150.014.1313871
TTGTGTT975350.013.0090023
TGTGTTT1057200.012.0528564
GTGTTTG1094450.011.6302735
CACGGTA108000.011.208837545
TGTTTGA1217300.010.4767086
CGGTACT66550.010.37988145
CTCACGG121650.09.9955843
TCACGGT123300.09.98770944
ACTTTGT625400.09.6244653
CCTCACG133250.09.14249542
ACGGTAC76900.09.04635744
CTACCCG60800.08.91561418
GAGGCTA59550.08.91448814
TGACGGG122600.08.89844510
CCCTCAC137150.08.84993241
TTTGAGG210750.08.8267378
Produced by FastQC (version 0.11.2)