FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l04n01 foxfdnnk418hpf2.34100000008fb5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l04n01 foxfdnnk418hpf2.34100000008fb5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43213808
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT821000.016.8657152
TAACTTT396500.015.87731
AACTTTG405250.015.4523852
CTTTGTG936600.014.8853651
TTGTGTT1043350.013.7542663
TGTGTTT1135450.012.6921154
GTGTTTG1178100.012.2000755
CACGGTA131750.011.51106345
TGTTTGA1314400.010.9382036
CTCACGG145500.010.369369543
TCACGGT150500.010.0525544
CTACCCG57400.09.95305818
GAGGCTA56500.09.83350614
CGGTACT85700.09.74090345
ACTTTGT659900.09.6461743
TGACGGG128550.09.53631910
CCTCACG159550.09.45638442
GCTACCC61150.09.41625517
TTTGAGG230350.09.4133388
CCCTCAC162050.09.296761541
Produced by FastQC (version 0.11.2)