FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l04n01 foxfdnnk418hpf1.34100000008f08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l04n01 foxfdnnk418hpf1.34100000008f08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35669660
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT334850.016.2328471
TTTGTGT689700.015.905472
AACTTTG354250.015.2453122
CTTTGTG771500.014.3185621
TTGTGTT872650.013.0502473
TGTGTTT943450.012.0876164
GTGTTTG973400.011.6879365
CACGGTA94700.010.95382245
TGTTTGA1074250.010.5862986
CGGTACT58100.010.10832245
ACTTTGT551950.09.9231633
CTCACGG105700.09.88513243
TCACGGT109100.09.61612444
TGACGGG114650.09.35827610
TTTGAGG184900.09.3184438
CCTCACG116350.09.07720242
CCCTCAC117750.08.89294241
ACGGTAC68100.08.59538144
GACGGGC93450.08.56883111
CTACCCG53650.08.46883118