FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l04n01 foxfcamras18hpf2.34100000008fc2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l04n01 foxfcamras18hpf2.34100000008fc2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40171756
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT399650.017.7083721
AACTTTG413050.017.0337922
TTTGTGT860200.016.4134542
CTTTGTG949850.014.9371761
TTGTGTT1096600.013.4021473
TGTGTTT1179800.012.4856034
GTGTTTG1225950.012.0118325
CACGGTA123800.011.88699245
TGTTTGA1352200.010.8835436
ACTTTGT668100.010.7397153
CGGTACT74950.010.68792245
CTCACGG138350.010.64485443
TCACGGT143500.010.35454144
CCTCACG151500.09.73592842
CCCTCAC153650.09.61461441
TTTGAGG226900.09.4672828
ACGGTAC86400.09.35447744
AAAGTGC162050.09.23229423
AAGTGCT167100.08.96695624
AGTGCTT169050.08.916923525
Produced by FastQC (version 0.11.2)