FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l04n01 foxfcamras18hpf1.34100000008f4c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l04n01 foxfcamras18hpf1.34100000008f4c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18916457
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT338850.015.8645272
AACTTTG168900.015.3944762
TAACTTT170850.015.1298071
CTTTGTG388150.013.9631361
TTGTGTT434450.012.9791973
TGTGTTT467150.012.1043384
GTGTTTG478250.011.7669035
CACGGTA48200.010.83113745
TGTTTGA531800.010.6072346
GAGGGGC20650.09.91216311
CTCACGG54050.09.74943743
CGGTACT30400.09.62283145
TCACGGT55450.09.54200244
ACTTTGT280450.09.3754553
CTACCCG25500.09.17305218
TTTGAGG93550.09.016388
CCTCACG58350.08.953942
CTAAGCG26800.08.82295143
CCCTCAC59500.08.78114341
GAGGCTA24850.08.6895114
Produced by FastQC (version 0.11.2)