FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l03n01 foxflacz20hpf2.34100000008efa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l03n01 foxflacz20hpf2.34100000008efa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34863910
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT293800.015.999881
AACTTTG313850.014.9647832
TTTGTGT576550.014.3963192
CTTTGTG670450.012.4235751
TTGTGTT741250.011.8378633
TGTGTTT793600.011.0796024
GTGTTTG818600.010.6944435
ACTTTGT507850.09.5444941
CGGTACT55100.09.43475345
TGTTTGA934650.09.3759886
CACGGTA83650.08.79735245
ACGGTAC63450.08.304563544
CTCACGG90050.08.17749643
TTTGAGG165800.08.1668188
TCACGGT94300.07.999552744
TGACGGG98300.07.573827310
CTACCCG42250.07.55955618
CCTCACG99650.07.525376342
CCCTCAC100800.07.41726641
GAGGCTA40900.07.20415914
Produced by FastQC (version 0.11.2)