FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l03n01 foxflacz20hpf1.34100000008e66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l03n01 foxflacz20hpf1.34100000008e66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36870522
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT320750.017.0435141
AACTTTG334500.016.2936152
TTTGTGT702600.015.9498192
CTTTGTG782900.014.3766961
TTGTGTT874500.013.4653323
TGTGTTT945300.012.4806164
GTGTTTG986250.011.9212595
ACTTTGT555200.011.0147941
CACGGTA98000.010.7930945
TGTTTGA1104150.010.6623196
TCACGGT113100.09.51718844
CTCACGG112800.09.50284543
TGACGGG105850.09.47734110
TTTGAGG196250.09.0429448
CCCTCAC120600.08.92580941
CCTCACG121200.08.7885542
TTGACGG127200.08.5055129
CTACCCG47700.08.440536518
AAAGTGC128200.08.42244323
TTTGAGT139050.08.3791748