FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l03n01 foxfdnnk420hpf2.34100000008f3f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l03n01 foxfdnnk420hpf2.34100000008f3f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38971648
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT350850.018.4698931
AACTTTG374400.017.3146692
TTTGTGT829400.015.618492
CTTTGTG866500.014.94409751
TTGTGTT1023650.013.1776693
TGTGTTT1113850.012.1266764
GTGTTTG1149100.011.7584855
CACGGTA99300.011.71683245
ACTTTGT594800.011.0084783
TGTTTGA1274350.010.6008016
CTCACGG111950.010.35975143
TCACGGT116850.010.0021744
CCTCACG122700.09.48890542
CGGTACT58600.09.37046545
TTTGAGG252300.08.7455178
CCCTCAC135800.08.65661441
TTTGAGC158900.08.2806748
TGACGGG119200.08.15156810
TCCGATC51350.08.1032537
CTTCCGA54300.08.0362515