FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l03n01 foxfdnnk420hpf1.34100000008e73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l03n01 foxfdnnk420hpf1.34100000008e73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30020446
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT283200.018.6194761
AACTTTG306500.017.2977542
TTTGTGT651500.015.6644442
CTTTGTG709700.014.4441281
TTGTGTT819900.013.0561593
TGTGTTT885000.012.1338424
CACGGTA85150.012.10510845
GTGTTTG920050.011.6471075
ACTTTGT500200.011.06421851
CTCACGG95600.010.78853743
TCACGGT97450.010.65271444
TGTTTGA1028450.010.4455066
CCTCACG105300.09.85896142
CCCTCAC106950.09.72809541
CGGTACT50350.09.38658545
TGACGGG103300.09.23215410
TTTGAGG178500.09.0725998
GACGGGC81700.08.86484811
TTTGAGT126950.08.8587988
AAAGTGC118350.08.7624723
Produced by FastQC (version 0.11.2)