FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l03n01 foxfcamras20hpf2.34100000008f72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l03n01 foxfcamras20hpf2.34100000008f72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24292650
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT216450.017.4523321
AACTTTG232250.016.2805542
TTTGTGT483550.015.9461772
CTTTGTG522850.014.7988381
TTGTGTT607050.013.2911963
TGTGTTT659000.012.2639114
CACGGTA62550.011.90865145
GTGTTTG680050.011.8710425
TGTTTGA749150.010.7758396
CTCACGG71600.010.56731443
ACTTTGT367450.010.4249393
TCACGGT73600.010.27967344
CCCTCAC80150.09.49645241
CCTCACG80550.09.44911842
CGGTACT38400.09.37671445
TTTGAGG131750.09.2361188
TGACGGG76450.09.17955710
AAAGTGC87850.08.85999523
TTGACGG88550.08.7634159
TGAGGGG41550.08.60737510
Produced by FastQC (version 0.11.2)