FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l02n01 handrnoggin20hpf2.34100000008f22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l02n01 handrnoggin20hpf2.34100000008f22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42053873
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT355900.015.7831511
AACTTTG369850.015.2342972
TTTGTGT697350.014.5564282
CTTTGTG796250.012.82782651
TTGTGTT897550.011.8734583
TGTGTTT965100.011.1216434
GTGTTTG995650.010.7488555
CACGGTA105200.010.26714445
TGTTTGA1111600.09.6862286
ACTTTGT603550.09.4435263
CTCACGG117950.09.37294243
TCACGGT119000.09.30887244
CGGTACT66250.09.00086345
TTTGAGG196050.08.5584738
CCCTCAC130200.08.37091241
CCTCACG130950.08.33969842
TGACGGG119350.08.02806610
CTACCCG66100.07.75808618
AAGTGCT148100.07.67175824
ACGGTAC78100.07.668484744
Produced by FastQC (version 0.11.2)