FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l02n01 handrnoggin20hpf1.34100000008e80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l02n01 handrnoggin20hpf1.34100000008e80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34972126
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT312600.015.2543211
AACTTTG336750.014.1872942
TTTGTGT675950.013.9461962
CTTTGTG712850.013.2838981
TTGTGTT855500.011.5818833
TGTGTTT911150.010.9559514
GTGTTTG933600.010.6539635
CACGGTA107500.010.02655545
TGTTTGA1048000.09.5166076
CTCACGG119250.09.19476743
TCACGGT123150.08.88491644
ACTTTGT551950.08.8393381
CGGTACT68250.08.57226145
CCCTCAC130050.08.34547341
GTTTGAG455400.08.24305257
CCTCACG130600.08.240972542
ACGGTAC77800.07.52393944
TGACGGG108200.07.462662710
AAAGTGC152400.07.38159323
GACGGGC79250.07.20876811
Produced by FastQC (version 0.11.2)