FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l02n01 handrlacz20hpf2.34100000008eed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l02n01 handrlacz20hpf2.34100000008eed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46658371
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT390900.015.5462291
AACTTTG393400.015.32930852
TTTGTGT755050.013.7485262
CTTTGTG889700.011.734241
TTGTGTT989000.011.1080923
TGTGTTT1058850.010.3986844
GTGTTTG1076400.010.1895015
CGGTACT82600.09.86169445
CACGGTA130750.09.6376145
TGTTTGA1209450.09.1242356
ACTTTGT698000.08.8782283
CTCACGG143400.08.77674443
TCACGGT145150.08.67055644
ACGGTAC94750.08.53036144
TGACGGG155000.08.24235210
CCTCACG158700.07.874259542
CCCTCAC159350.07.856673741
TTGACGG179700.07.7602939
GACGGGC122700.07.644093511
ACGGGCG116550.07.29476412
Produced by FastQC (version 0.11.2)