FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l02n01 handrlacz20hpf1.34100000008e5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l02n01 handrlacz20hpf1.34100000008e5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31666942
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT297750.017.595551
AACTTTG326750.016.142612
TTTGTGT708500.014.6958222
CTTTGTG709750.014.6520851
TTGTGTT887050.012.2373053
TGTGTTT944050.011.5055874
GTGTTTG983150.011.0687555
ACTTTGT516500.010.2992923
TGTTTGA1113450.09.8056176
CACGGTA110400.09.41670945
GTTTGAG505600.08.6390667
CTCACGG127200.08.35484743
TCACGGT127750.08.21263544
CCTCACG134150.07.821607642
CTACCCG56300.07.750265618
CCCTCAC136350.07.69585641
GAGGCTA55350.07.68007314
CGGTACT69250.07.50617145
TTTGAGG194950.07.30310968
TTTGAGT180500.07.10272558
Produced by FastQC (version 0.11.2)