FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l02n01 handrdnfgfr20hpf2.34100000008f65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l02n01 handrdnfgfr20hpf2.34100000008f65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33159007
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT301650.017.1731551
AACTTTG301750.017.1075922
TTTGTGT678200.015.6080122
CTTTGTG752600.014.1404481
TTGTGTT855100.012.9683123
TGTGTTT909100.012.2499614
GTGTTTG954750.011.66435
ACTTTGT534700.010.5053821
CACGGTA94000.010.41306745
TGTTTGA1079150.010.35078056
CTCACGG105850.09.16772943
TCACGGT107050.09.14869144
CGGTACT58850.08.87071745
TTTGAGG189250.08.8184598
CCTCACG115400.08.56545642
CTACCCG44900.08.31558718
GAGGCTA42600.08.23640414
CCCTCAC116800.08.23188241
TGACGGG113050.08.15714110
TTTGAGC131450.07.8366338
Produced by FastQC (version 0.11.2)