FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l02n01 handrdnfgfr20hpf1.34100000008eaa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l02n01 handrdnfgfr20hpf1.34100000008eaa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35283961
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT329000.018.0269531
AACTTTG337800.017.4992852
TTTGTGT741850.014.6418392
CTTTGTG817900.013.3845411
TTGTGTT943050.011.9926493
TGTGTTT1001300.011.3174484
GTGTTTG1043950.010.8530035
CACGGTA114900.010.10531445
ACTTTGT604500.09.9536433
TGTTTGA1176550.09.6622076
CGGTACT72950.09.31540745
TCACGGT131550.09.03651444
CTCACGG130950.08.97519443
GAGGCTA64100.08.49144714
CCTCACG141900.08.31468142
CTACCCG67900.08.31455118
CCCTCAC140750.08.27104641
CTAAGCG68700.08.02949443
ACGGTAC85900.07.994007644
GTTTGAG565300.07.97342637
Produced by FastQC (version 0.11.2)