FastQCFastQC Report
Fri 14 Nov 2014
lane6_Undetermined_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane6_Undetermined_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4512823
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT1652.404704E-816.34062417
ACTACGT2002.4873407E-713.50360936
ACGTATA2002.5447662E-713.47832418
TAAGCCG1902.0635543E-613.02719722
GACTACG2309.806172E-812.72262835
CGAGACC1651.01322905E-412.31236430
GTTCGAC1300.00305057712.1011611
CGTATAG2251.0405074E-612.00067719
CTCGTAA2251.0560943E-611.9859131
CGGAATA2005.054388E-511.23442652
CGCAAAC2604.934809E-711.23442652
ACATCGA2251.3856281E-510.9827034
ATGTTCG1450.0069101210.844995
CATCGAA3351.0064468E-810.7293715
GCGCAAA2108.283541E-510.7017081
GCGGAAT2108.283541E-510.7017081
GTGCGTT1903.9138683E-410.6453831
GTCCTAT3053.4339246E-710.3157441
CTCGAAT5201.8189894E-129.940241
CGTATCT2952.5081335E-69.8997743
Produced by FastQC (version 0.11.2)