FastQCFastQC Report
Fri 14 Nov 2014
PM3_GTGAAA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePM3_GTGAAA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16371061
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT14850.016.5155217
ACGTATA15700.015.47564118
CGTATAG16250.014.9518519
ACTACGT17250.013.82660636
GACTACG18050.013.4620435
AGACGTA20450.012.21302416
CTACGTC20250.012.11156637
ACGTCTA21750.011.58830539
AATCCGA22600.011.54730724
TCGTATC12200.011.4355892
CGTATCT12700.011.1605413
CTCGTAT19050.010.987721
ATCCGAA24200.010.87707825
ACGTTCG5750.010.56380756
CGTGCGA5350.010.51505638
GGGGTAT25150.010.2020251
AAATCCG25550.010.12600523
GTCGGAT9350.010.1101421
CTCGAAT40000.09.509251
TCGTTTT42100.09.4071332
Produced by FastQC (version 0.11.2)