FastQCFastQC Report
Fri 14 Nov 2014
PM2_CCGTCC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePM2_CCGTCC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15246486
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTC10950.018.08011832
CGTCCCG12600.015.89223235
GATCGGA13750.015.3864621
ATCCGAA30800.015.19341225
GACGTAT16100.014.95385817
AATCCGA32800.014.67809824
CGTATAG16150.014.62642119
ACGTATA17150.013.90474518
AAATCCG33900.013.86999923
ATCGGAA16350.012.5244572
ACACGTC18000.012.25136813
AGACGTA19300.012.24133516
GTCGCAA4250.012.1801471
GACTACG19250.012.03848935
CGTCTGA17900.011.816030516
GCGGCTT40700.011.77649915
TCCGAAG41100.011.71425326
ACTACGT19300.011.65818536
ACGTCTG19550.011.2800315
TATCATG43800.011.0436
Produced by FastQC (version 0.11.2)