FastQCFastQC Report
Fri 14 Nov 2014
PA3_CTTGTA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePA3_CTTGTA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21144206
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT12150.014.81503717
GTACACT88900.013.8989781
AATCCGA29400.012.77901924
ACGTATA15350.011.72467318
GACTACG15200.011.69353535
CGTATAG15250.011.21147819
GTCGTTT28650.010.9194931
AGACGTA18400.010.51648616
ATCCGAA36250.010.30267625
ACTACGT18550.010.06758436
CTCGTAT35650.09.9117981
ACTGTAG126400.09.8249345
TACACTG114700.09.7303812
GTCGGTT27500.09.6574311
AAATCCG37250.09.54236723
GTCAGTA54200.09.5092981
GTCGCAT9000.09.5028391
GTCCAAT39650.09.422741
TATCATG41100.09.3052566
TGTAGCT148500.09.18057
Produced by FastQC (version 0.11.2)