FastQCFastQC Report
Fri 14 Nov 2014
PA2_CAGATC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePA2_CAGATC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20506376
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT15950.015.23571817
GTACACT77350.013.7638691
ACGTATA18350.013.36304918
CGTATAG18350.013.24045219
AATCCGA32050.012.91550224
GACTACG19850.012.12788335
AGACGTA22050.011.42908416
ACTACGT21600.011.04210836
ATCCGAA38950.010.80126725
AAATCCG39600.010.56667123
TACGAGG18400.010.3924096
CCGATCG7450.010.2674893
ACGTCTA25450.010.08075839
TTCGACG14550.09.8981232
CGATAAG10700.09.88530840
GGTACGA19900.09.8356614
TGTAGCT124900.09.7262737
CGTCTAC24950.09.6513840
ACTGTAG115450.09.6070575
TATCATG45600.09.5215496
Produced by FastQC (version 0.11.2)