FastQCFastQC Report
Fri 14 Nov 2014
LM3_GCCAAT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLM3_GCCAAT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15201960
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGA38600.018.88345524
AAATCCG40950.017.96448523
ATCCGAA40800.017.81091125
GACGTAT18200.016.19542117
TATCATG45200.016.1757496
ACGTATA19000.015.62925918
GCGGCTT48300.015.18822915
CGTATAG20250.014.77558419
TCCGAAG50550.014.55364726
GACTACG20050.014.25134935
CCGAAGT51300.014.20930627
ATCATGC54650.014.2019647
AGACGTA21350.014.12219316
TTACGGC17350.013.61939840
ACTACGT21050.013.57508736
ACGTCTA22200.013.48234339
CTACGTC21150.013.40451537
GTATCAT56650.013.3435615
TAGAACG26600.013.10904422
ATTACGG19200.013.01033339
Produced by FastQC (version 0.11.2)