FastQCFastQC Report
Fri 14 Nov 2014
LM2_TGACCA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLM2_TGACCA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17990949
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA17200.012.5619551
TCGTATG20300.011.86304443
AATCCGA30750.011.6328324
ATCCGAA30200.011.54689925
AATCTCG20200.010.80677739
CTCGTAT21800.010.42713242
AAATCCG33250.010.41981523
ACGTATA15750.010.14145718
ATCGGAA20900.010.1207232
CGTATAG16100.010.06072119
GACGTAT15450.010.04869217
CGTATGC23300.09.65950644
ACGTCTG24600.09.33005315
TCGTATC11100.09.3253432
ACACGTC24350.09.14866513
GTCGGAT10250.09.0027351
CGTCTGA26100.08.96564916
GTCGGTT27750.08.9216291
GCGGCTT40950.08.736999515
AGACGTA19000.08.52644216
Produced by FastQC (version 0.11.2)