FastQCFastQC Report
Fri 14 Nov 2014
LA3_ACAGTG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLA3_ACAGTG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19270284
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACT77100.013.25322151
GGGATTT69150.010.5128745
ACTGTAG114000.010.005675
TACACTG96900.09.8463752
TGTAGCT131750.09.2549417
ACACTGT106850.09.2434753
GTCGGTT25500.09.1793761
CACTGTA127450.09.1792354
TAGCTGT132550.09.0639399
TGGGATT87500.08.41099344
GTCCTAT18550.08.1292581
GTCGCAT8050.08.1081561
CCCGTAT18350.08.0952051
GTAGCTG155200.07.94353538
GTCGAGT13200.07.84339141
CAATCGA8050.07.8253754
GTCGGAT20500.07.6853591
CTGTAGC139700.07.63322456
TGGTTGC105400.07.621104737
GTGCATT41650.07.51138071
Produced by FastQC (version 0.11.2)