FastQCFastQC Report
Fri 14 Nov 2014
LA2_CGATGT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLA2_CGATGT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15349612
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACT60750.013.4862011
GACGTAT7150.011.95825417
AATCCGA20250.010.88776924
TGTAGCT95000.09.851337
ACTGTAG87100.09.8155495
GTCGCAT6250.09.7234031
CTCGAAT35200.09.7193111
TACACTG81550.09.4371712
GTCGTTT21300.09.2990281
CGTATAG9300.09.19233319
GCCGTTA2453.8600192E-49.1868891
ACACTGT84300.09.1275323
ACGTATA9650.09.09206218
ACGCTTA7700.09.06072743
GTGCATT34800.08.9902151
ATTGCGC9300.08.95174136
GACTACG10650.08.87273135
GTCGCTT27650.08.7915041
TAGCTGT101700.08.6940229
GTCCTAT18200.08.6568771
Produced by FastQC (version 0.11.2)