FastQCFastQC Report
Fri 14 Nov 2014
series_92V5_200_Undetermined_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameseries_92V5_200_Undetermined_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84833866
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGAGT7646350.044.5714721
TCGAGTG2891600.044.311692
TCGAGTC2574650.044.2173042
TTACGCT4005100.044.0583421
AGTCTAT4439350.043.8513571
CGAGTGT999450.043.757283
TACGCTC1823950.043.7395972
CGAGTCT1042100.043.624613
CGAGTCG540100.043.5169033
GTCTATG2069050.043.3419462
TCGAGTA1637400.043.3101352
CTCAGAT3122350.043.194331
CGAGTCC522150.043.0436253
TACGCTG1242800.042.9762572
CGAGTGC738400.042.8029823
ACTTATG1861000.042.7986762
GACTTAT4205450.042.5823631
ACGCTCC650350.042.429423
GCTCCCG222500.042.3604135
ACAAGTT3824950.042.2643621
Produced by FastQC (version 0.11.2)