FastQCFastQC Report
Fri 14 Nov 2014
series_92V1_200_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameseries_92V1_200_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93825955
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCT4375850.044.195861
AGTCTAT5138150.044.1524931
TACGCTC1975450.043.9939842
GAACGTT7515700.043.691531
GTCTATG2165150.043.4804152
CTCAGAT3598600.043.318741
GACTTAT5434800.043.247421
GTGGCCT4856800.043.2096141
TACGCTG1300250.043.1100732
GATCAGT4077950.043.0019341
ACTTATG2120050.042.9185142
ACGCTCC669500.042.7849123
ACAAGTT5142650.042.76021
TCACAGT3431900.042.435011
GACCATG1889150.042.4342772
TAGCTTT6586350.042.2539371
GCTCCCG246900.042.2170145
TGAAACT3668000.042.032181
GTCTATC1629100.041.951562
CACAGTG1123950.041.9168432
Produced by FastQC (version 0.11.2)