FastQCFastQC Report
Fri 14 Nov 2014
lane4_Undetermined_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane4_Undetermined_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4493015
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAA118270.26323081494275No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC109100.24282135715104447No Hit
GTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTA82530.18368512012535013No Hit
ACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTTGA67730.15074510100678498No Hit
GTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTTGAAG64660.1439122727166502No Hit
AATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTTGAAGAG63620.14159756867048073No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG61040.13585532209440654No Hit
CTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAACAAATTTC54820.1220116113567393No Hit
GACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTTG54630.12158873273291988No Hit
GAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAGTTA50780.113019876408158No Hit
CTTGTCAGTGACACCGTACTTCTTAGCGACAGTGGCAGTGTCACCACCACC47660.10607576426964968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG4150.030.30428511
CCCGGGT554.2216525E-628.589771
GCGTGGT12250.027.31551717
GTGCCTT9150.027.0051671
GCCCCGT3700.026.713221
GCCGGTT4100.026.2986011
GCGCCCG1200.026.2072871
GGTCGTG12800.025.65858321
GGCCGCT2050.025.2028271
CACACGT4850.025.00438112
CTCCCCG901.2138116E-824.9593221
CGCGCGC1350.024.959321
GTCGTGT13100.024.8992622
ACACGTC4900.024.74923313
CCTCGGG1550.024.6309615
GTGTCAC10050.024.15428438
GGCGCGC851.9262552E-723.7847651
CGGGCGG7750.023.7677171
GAACTCC5200.023.36039521
CGTCTGA5500.022.86596116
Produced by FastQC (version 0.11.2)