FastQCFastQC Report
Fri 14 Nov 2014
Hyb_T9_ACTTGA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHyb_T9_ACTTGA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12457123
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGGCAGACATT506070.4062495007876216No Hit
CTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAACAAATTTC450420.36157626443922886No Hit
CTTGTCAGTGACACCGTACTTCTTAGCGACAGTGGCAGTGTCACCACCACC438850.35228840559734376No Hit
CAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGACCAAAGTGG312340.25073205105223734No Hit
AGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAAATTAATTT288220.23136963486673445No Hit
GACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGACCAAAGT244090.19594411968156694No Hit
AGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGGCAGACATTCT225410.18094868293425376No Hit
CTTCACCAGCGTATCTACCGTGAGTGGAGTCGTACTTGAACATGTAAGCAG220100.17668606146057964No Hit
CAACAGCGTCTTCGGTGTAACCCAAAACACCCTTCAACTTACCTTCAGCGG200300.1607915407112862No Hit
GTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAGCAGTGTCT198770.15956332774429538No Hit
GTCTTGGACTTAGACAAGTCAGCCAAATGTTGGTACAATGGGACGTTCTTT196580.15780529741899474No Hit
GTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGACCAAAGTGGTA185920.1492479443287186No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC170720.13704609001612972No Hit
GTGCAAGACAATCATTTCCAATAAGGCATCGGCAGCTGGCAAGAACTTTCT139930.1123293074974053No Hit
TAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGGCAGACATTC136430.10951966999121707No Hit
TAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGCTTCCAACATACC135640.10888549466839173No Hit
GTCAACGTGACCTGGAGAGTCGATCAAGTTGATCAAGAAGGAGTTACCGTC128330.10301736604832433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGTG73550.033.79910321
GTCGTGT76250.032.9268322
GCGTGGT76300.032.70476517
AGCGTGG78100.032.5561616
CAGCGTG85600.029.75626615
TGGTCGT84200.029.60419320
TCGTGTT85750.029.3314323
CGACGTC35150.029.26228345
GTGACAC79900.028.9448918
GTGTCAC82300.028.51850938
GTGGTCG100150.027.83209619
CCGTACT84250.027.82938214
GGAACAC91350.027.6810998
ACCGTAC85150.027.50881213
TTAGCGA85650.027.29064223
TAGCGAC86200.027.11651424
ACACCGT89250.025.94256611
TTGTCAG92800.025.4105342
ATCTTGC101350.024.8624734
CAGAGCG12350.024.77407833
Produced by FastQC (version 0.11.2)