FastQCFastQC Report
Fri 14 Nov 2014
Hyb_T7_ATCACG_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHyb_T7_ATCACG_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18521149
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAACAAATTTC385460.20811883755160113No Hit
GTAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGGCAGACATT366030.19762812771497057No Hit
CTTGTCAGTGACACCGTACTTCTTAGCGACAGTGGCAGTGTCACCACCACC343020.18520449244266649No Hit
CAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGACCAAAGTGG267590.14447807746700814No Hit
GACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGACCAAAGT233670.12616387892565412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGT69650.031.20265822
AGCGTGG73100.030.4110516
GCGTGGT73200.029.57006817
GGTCGTG73150.029.46366321
CAGCGTG81050.027.34482215
GTGGTCG93100.025.61484519
TCGTGTT88450.025.02838523
GCCGGTT23100.024.649511
GTGACAC70250.023.6664818
GCCGGAA23950.023.3987981
GTGTCAC71100.023.38872538
CGGCTTA20400.023.2733762
CGCTCCG39650.023.0933418
TGGTCGT94250.023.05851620
GCCCCGC6450.023.0291645
ACCGTAC74350.022.87876913
CCGTACT75800.022.44111614
GGAACAC98150.022.3027348
CGACGTC31250.021.96563145
TAGCGAC75650.021.7689624
Produced by FastQC (version 0.11.2)