FastQCFastQC Report
Fri 14 Nov 2014
Hyb_T6_CTTGTA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHyb_T6_CTTGTA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18698171
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAACAAATTTC324190.17338059428379385No Hit
CTTGTCAGTGACACCGTACTTCTTAGCGACAGTGGCAGTGTCACCACCACC292260.15630405776051573No Hit
GACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGACCAAAGT258730.1383718225702396No Hit
GTAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGGCAGACATT255230.13649998173618158No Hit
CAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGACCAAAGTGG225410.12055189783000701No Hit
GAGAAAAGAAAAAAATTGATCTATCGATTTCAATTCAATTCAATTTATTTC201940.10799986800848062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGT62450.029.72021122
AGCGTGG64300.028.9395816
GCGTGGT64200.028.31867217
GGTCGTG66150.027.81978821
CAGCGTG71000.026.27204715
GCCGGAA26550.025.4305381
CGGCTTA19750.024.6088522
GTGGTCG85350.024.54011219
GCCGGTT24500.024.5269741
GCCCCGC6100.024.35149645
ACTCCAG26000.023.7952223
GAACTCC26650.023.468121
GTATGCC26600.022.76052345
GTGACCG33750.022.6727351
TCGTGTT83200.022.63249823
GCCCGCT11550.022.6034531
GCACACG26750.022.54284511
GTGTCAC67650.021.92089838
ACACGTC28450.021.67035513
TGGTCGT86200.021.27064720
Produced by FastQC (version 0.11.2)