FastQCFastQC Report
Fri 14 Nov 2014
Hyb_T5_CAGATC_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHyb_T5_CAGATC_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13586413
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAACAAATTTC226440.16666650719362056No Hit
CTTGTCAGTGACACCGTACTTCTTAGCGACAGTGGCAGTGTCACCACCACC194030.14281179292871488No Hit
GTAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGGCAGACATT160450.11809592421487555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGT42200.028.36127722
GGTCGTG42550.027.9693721
AGCGTGG44150.027.92834516
GCGTGGT44250.026.89899617
GCCGGAA20400.025.7045861
CAGCGTG49000.024.98032815
GTGGTCG54950.024.7283919
GTCCGGT4400.023.0167851
GTGTCAC44350.022.57958838
TCGTGTT55150.022.10957123
GCCGGTT15900.021.6560441
GCCCCGC3450.021.52855345
GTGACCG23100.021.433621
CGCTCCG22450.021.34468818
CCCGGGC1900.021.3208121
TGGTCGT57300.020.84809920
CCGTACT49800.020.73856414
ACCGTAC50050.020.59001713
CGGCTTA15000.020.550832
GTGACAC50100.020.5213098
Produced by FastQC (version 0.11.2)