FastQCFastQC Report
Fri 14 Nov 2014
Hyb_T4_GCCAAT_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHyb_T4_GCCAAT_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14638818
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTCAGTGACACCGTACTTCTTAGCGACAGTGGCAGTGTCACCACCACC176040.12025561080136389No Hit
CTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAACAAATTTC170560.11651213916314827No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC164580.11242710989370862TruSeq Adapter, Index 6 (100% over 51bp)
GTAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGGCAGACATT156400.10683922704688316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC23650.036.2431729
TCACGCC26300.032.9334630
GCACACG27000.032.83582311
ACACGTC29050.030.13137413
ACTCCAG29500.029.97050323
CACACGT29850.029.70074512
TATCTCG29200.029.36441839
CACGCCA30150.029.10111631
GAACTCC31100.028.50094821
GAGCACA33350.027.5224939
AGCACAC35550.026.51547410
CGTATGC34450.025.47937444
GTATGCC34600.025.43361545
GTCGTGT38850.025.18962522
GGTCGTG37550.025.10311121
AGCGTGG37950.025.08095416
CGTCTGA35550.025.00189616
TCTCGTA34750.024.80510341
GCCGGTT19250.024.668591
ACGTCTG35450.024.62810315
Produced by FastQC (version 0.11.2)