FastQCFastQC Report
Fri 14 Nov 2014
Hyb_T3_ACAGTG_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHyb_T3_ACAGTG_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13286794
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC465830.35059623864116507TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG62350.037.0998111
GAACTCC62950.036.95302221
ACACGTC63200.036.74325613
GTCACAC62500.036.49945429
ACTCCAG64200.036.12840323
GTATGCC64850.035.1920345
CGTCTGA67900.034.66386416
CACACGT66700.034.61278512
ACGTCTG69500.033.86584515
TCACACA67550.033.80407730
GAGCACA70500.033.6657649
CACGTCT69850.033.34179714
CGTATGC68000.033.33012444
TCTCGTA68350.033.28991341
AGCACAC72400.032.8445410
GATCTCG69250.032.66042339
ATCTCGT70800.032.07254440
AGTCACA73250.031.29644228
CACACAG73500.031.06755631
CAGTCAC74150.031.00759927
Produced by FastQC (version 0.11.2)