FastQCFastQC Report
Fri 14 Nov 2014
Hyb_T2_TGACCA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHyb_T2_TGACCA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14597387
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC283530.19423339259279762TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCC45600.032.3144821
GCACACG44100.032.2474911
ACTCCAG46150.031.5393923
ACACGTC45950.030.9491713
CACACGT47000.030.01837212
GTATGCC45550.029.84459545
CGTATGC46700.029.2547544
CGTCTGA51100.028.57861716
CACTGAC48900.028.5697531
ACTGACC49350.027.85327532
ACGTCTG53200.027.45051615
TCTCGTA50500.027.40873141
AATCTCG50750.027.13974439
AGCACAC53650.027.130910
GAGCACA54150.026.9213779
ATCTCGT51500.026.8757940
GTCACTG53650.026.33380529
AGTCACT54300.026.01857828
CACGTCT55200.025.76294114
TCGTATG53400.025.6262743
Produced by FastQC (version 0.11.2)