Basic Statistics
Measure | Value |
---|---|
Filename | Hyb_T1_CGATGT_L004_R1_001.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 14526305 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTGCCTTTTGGTAACGACTGATCGATAGATACGACCTTTAAAACGCCGTCT | 51956 | 0.3576683816015153 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC | 33171 | 0.2283512565652449 | TruSeq Adapter, Index 2 (100% over 51bp) |
GCCTTTTGGTAACGACTGATCGATAGATACGACCTTTAAAACGCCGTCTGC | 28983 | 0.1995208003687104 | No Hit |
GCCGTCTGCTTCTCCTGCAAGGATCATACCTTGAGAAAGCTCTCCTCTGAG | 21735 | 0.1496251111345934 | No Hit |
GTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGAT | 17115 | 0.11782073968569433 | No Hit |
GCCGGTCATTGGGAATTTCCTTCACTACTTCCTTCGGTTTCTTCGCATTTT | 15820 | 0.10890587799168475 | No Hit |
GCTGAGTCAACAGCAGAGAGATGCTCCACAGGAGTTTGCTCTGCAATAGCA | 15624 | 0.107556601627186 | No Hit |
CTTTTCGATACGAGTAATTGTAATATCAGCCTTTCCTTTCGTCAGCTTTGC | 14996 | 0.10323341001032264 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATCTCG | 4720 | 0.0 | 35.713196 | 39 |
GAACTCC | 5180 | 0.0 | 33.788803 | 21 |
TCGCCCG | 6440 | 0.0 | 33.2602 | 2 |
GTATGCC | 5145 | 0.0 | 33.203865 | 45 |
GTGCCTT | 9310 | 0.0 | 32.971268 | 1 |
CTCGCCC | 6620 | 0.0 | 32.533062 | 1 |
CGTATGC | 5375 | 0.0 | 31.448378 | 44 |
CCGATGT | 5390 | 0.0 | 31.220314 | 33 |
TCTCGTA | 5470 | 0.0 | 30.900816 | 41 |
CGTCTGA | 5715 | 0.0 | 30.749638 | 16 |
CAGTCAC | 5650 | 0.0 | 30.699646 | 27 |
ATCTCGT | 5515 | 0.0 | 30.565372 | 40 |
CGCCCGG | 7005 | 0.0 | 30.544266 | 3 |
ACTCCAG | 5915 | 0.0 | 30.236767 | 23 |
ACACGTC | 5805 | 0.0 | 29.575186 | 13 |
ACGTCTG | 6110 | 0.0 | 28.651249 | 15 |
GCACACG | 6205 | 0.0 | 27.741173 | 11 |
AACTCCA | 6495 | 0.0 | 27.190266 | 22 |
TCGTATG | 6295 | 0.0 | 27.138311 | 43 |
CTCGTAT | 6355 | 0.0 | 26.739681 | 42 |