FastQCFastQC Report
Fri 14 Nov 2014
Hyb_T1_CGATGT_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHyb_T1_CGATGT_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14526305
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCTTTTGGTAACGACTGATCGATAGATACGACCTTTAAAACGCCGTCT519560.3576683816015153No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC331710.2283512565652449TruSeq Adapter, Index 2 (100% over 51bp)
GCCTTTTGGTAACGACTGATCGATAGATACGACCTTTAAAACGCCGTCTGC289830.1995208003687104No Hit
GCCGTCTGCTTCTCCTGCAAGGATCATACCTTGAGAAAGCTCTCCTCTGAG217350.1496251111345934No Hit
GTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGAT171150.11782073968569433No Hit
GCCGGTCATTGGGAATTTCCTTCACTACTTCCTTCGGTTTCTTCGCATTTT158200.10890587799168475No Hit
GCTGAGTCAACAGCAGAGAGATGCTCCACAGGAGTTTGCTCTGCAATAGCA156240.107556601627186No Hit
CTTTTCGATACGAGTAATTGTAATATCAGCCTTTCCTTTCGTCAGCTTTGC149960.10323341001032264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG47200.035.71319639
GAACTCC51800.033.78880321
TCGCCCG64400.033.26022
GTATGCC51450.033.20386545
GTGCCTT93100.032.9712681
CTCGCCC66200.032.5330621
CGTATGC53750.031.44837844
CCGATGT53900.031.22031433
TCTCGTA54700.030.90081641
CGTCTGA57150.030.74963816
CAGTCAC56500.030.69964627
ATCTCGT55150.030.56537240
CGCCCGG70050.030.5442663
ACTCCAG59150.030.23676723
ACACGTC58050.029.57518613
ACGTCTG61100.028.65124915
GCACACG62050.027.74117311
AACTCCA64950.027.19026622
TCGTATG62950.027.13831143
CTCGTAT63550.026.73968142