FastQCFastQC Report
Fri 14 Nov 2014
Hyb_T12_GGCTAC_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHyb_T12_GGCTAC_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9693040
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAA1399121.4434274489736967No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC1342641.3851588356181344No Hit
CCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTG920940.9501044048100492No Hit
GTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTA821040.8470407632693149No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC755040.7789506697589198No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG727510.750548847420417No Hit
ACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTTGA623240.6429768163548278No Hit
AATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTTGAAGAG589340.6080032683244885No Hit
GAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAGTTA585940.6044955968406197No Hit
GTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTTGAAG577540.5958295849392966No Hit
GACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTTG528740.5454841824649439No Hit
GCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAG473210.4881956537886979No Hit
ATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAGTTATTA472000.48694733540767393No Hit
GCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTC432210.4458972623655737No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT416390.4295762732847487No Hit
GGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGT391650.4040528048991854No Hit
GCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCA384710.396893028399759No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAA384700.39688271171892414No Hit
GCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT374680.38654539752234596No Hit
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGC339730.35048859800434123No Hit
GCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTCGCCAA330190.3406464844878387No Hit
CAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAGT320700.3308559543755107No Hit
GCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATA309720.31952823881878134No Hit
CCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTGA305700.31538093312314813No Hit
GCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAA304490.3141326147421243No Hit
GGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTT274250.28293497189736144No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTT272600.28123271955960155No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTG266430.2748673274844631No Hit
GCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCA254070.2621159099725163No Hit
GCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCAT239330.24690912242186147No Hit
AGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAGTT231190.23851134422224607No Hit
CCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAA228440.23567425699264627No Hit
GTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTAC227400.23460132218581578No Hit
CTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAACAAATTTC222230.22926759819416817No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGC220960.22795737972813482No Hit
GGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTC197970.20423933048868054No Hit
GCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTATTT197020.20325924580936428No Hit
GGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCC182570.1883516420029217No Hit
GTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTGATAGGGCAGA181620.1873715573236054No Hit
AGCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCC174950.18049033120672153No Hit
CAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTC174110.1796237300165892No Hit
GTCCTAGAAACCAACAAAATAGAACCAAACGTCCTATTCTATTATTCCATG173430.17892219571981546No Hit
CTAGAAACCAACAAAATAGAACCAAACGTCCTATTCTATTATTCCATGCTA172710.17817939469970207No Hit
ACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGG161990.1671199128446803No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCG158230.16324084085075477No Hit
GCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCT158220.16323052416991984No Hit
GTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCC156210.1611568713221033No Hit
CTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAGTTT151510.15630803132969637No Hit
GCATGTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAACT145010.14960218878700593No Hit
CCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTA142090.1465897179832127No Hit
CCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCATC137900.1422670287133861No Hit
GGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCA136740.14107029373653673No Hit
CCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAAA135620.139914825483027No Hit
ATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTTGAAGAGC134020.13826415654944166No Hit
GTCGGCATAGTTTATGGTTAAGACTACGACGGTATCTGATCATCTTCGATC131190.13534453587316259No Hit
AGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC130130.13425096770466233No Hit
GTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAATAGAACCAAA124980.12893787707468451No Hit
GCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGT124740.12869027673464672No Hit
CGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCC123580.12749354175779737No Hit
TAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGCTTCCAACATACC122420.12629680678094798No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAGAA120600.12441917086899465No Hit
CTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTCGCCAAGA115070.11871404636729034No Hit
CAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACC114350.11797124534717693No Hit
CTCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTC112290.11584600909518583No Hit
GCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTT111780.1153198583726055No Hit
GCCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTGATAGGG111270.11479370765002518No Hit
CAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTTGAAGAGCAA107740.11115191931530254No Hit
AACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTTGAAGAGCAAT101660.10487937736767824No Hit
GGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC98450.10156772281967266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGC6650.040.951861
GTCCCCG4250.037.5992621
GCCCCGT21400.037.1253741
CCGGGCG4300.036.638651
GCGCCCG4250.036.5401271
GGCGCGC3450.036.532451
CGTCCCG250.002109169535.9955148
ACGGCTA9850.035.63007732
CGGCTAC9800.035.12317733
GCGGGCG4650.033.8809051
GGTCGTG33250.033.69505321
CACGGCT10600.033.53355831
GGCAGAA208150.033.367941
GTCGTGT33850.033.09779722
GGCGCCT17750.032.8406141
AGCGTGG35000.032.7227516
CGCGGTG5050.032.534291
GCGTGGT34650.032.46884517
CCCCCGT10000.032.4095041
GCACACG11000.031.91038311
Produced by FastQC (version 0.11.2)